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Mullane says that this sublingual liquid is ideal for vegans and contains three forms of B12 in an orange-flavoured liquid. The complex contains methylcobalamin, hydroxocobalamin and adenosylcobalamin three biologically active forms of B12

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After electroporation, cells were transferred into 1 mL LB medium without antibiotics, shaken at 30 C for 1 h, and plated onto an LB agar plate containing tetracycline (3 g mL 1 ) and/or spectinomycin (100 g mL 1 ) to obtain transformants